Table 1. Genes associated with MNase-sensitivity.
Treatment/group | ♂ [n] | Total genes [n] | MAT+ genes [n] | MAT− genes [n] |
PargKO | 10 | 11,777 | - | - |
Wildtype | 9 | 14,060 | - | - |
PargA/Wt | 1 | 4,877 | 2,320 | 3,027 |
PargB/Wt | 1 | 4,854 | 2,301 | 3,022 |
PargC/Wt | 1 | 4,922 | 2,148 | 2,957 |
All Parg/Wt | 10 | 5,148 | 2,501 | 3,233 |
PJ34 | 4 | 14,787 | - | - |
PJ34A/Wt | 1 | 6,063 | 3,700 | 3,089 |
PJ34B/Wt | 1 | 7,740 | 4,039 | 4,773 |
PJ34C/Wt | 1 | 5,945 | 2,567 | 4,077 |
All PJ34/Wt | 4 | 7,839 | 4,582 | 4,791 |
Parg(110)−/− (PargKO), PJ34, Wildtype controls: groups of sperm samples analyzed for gene histone association by tiling arrays with genomic “input” correction. Numbers of genes with significant histone binding in sperm are indicated. ♂ [n]: numbers of males. PargA/Wt, PJ34A/Wt, etc.: sperm samples of single males (♂, n = 1) analyzed for sperm histone association after pair-wise comparison to wild-type samples. Total genes [n]: number of sperm genes (differentially) associated with histones, indiscriminate whether sensitivity was increased (MAT+ genes [n]) or decreased (MAT− genes [n]). Please note that a number of genes were associated with both (MAT+ and MAT−) fractions. All Parg/Wt, All PJ34/Wt: comparison of all Parg(110)−/− males (n = 10) with wild-type (n = 9) males or males treated with PJ34 (n = 4) with wild-type control males (n = 9).