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. 2014 May 8;10(5):e1004115. doi: 10.1371/journal.ppat.1004115

Figure 3. Acid-fast staining of M. tuberculosis kasB isogenic mutant strains.

Figure 3

(A) Cultures were fixed on glass slides and acid-fast staining was performed on the fixed smears using either the BD TB Auramine Kit or the BD Carbolfuchsin kit. The left panels show phase contrast microscopy images of Mtb CDC1551 parental strain and the phosphomimetic and phosphoablative Mtb isogenic strains. (B) Restoration of the acid-fast staining phenotype in the ΔkasB and phosphomimetic KasB mutant strains complemented with pMV261::kasB. Magnification = 100×.