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. 2014 May 8;10(5):e1004115. doi: 10.1371/journal.ppat.1004115

Figure 7. Infection of C57BL/6 bone marrow-derived macrophages with M. tuberculosis kasB variants. (A) Growth of the KasB mutants and parental strain in BMDM.

Figure 7

An MOI of 1 was used to infect the BMDM with the strains. Macrophages were lysed after 0, 1, 3 and 6 days post-infection and viable bacteria were counted by plating dilutions of the lysates on agar plates. Three independent experiments were performed and each experiment was done in triplicate. (B) Uptake of KasB strains by BMDM. The ratio between the bacterial titer after a 4 h infection and the inoculum titer is shown. A T-test (two-tailed distribution, unequal variance) was performed and showed a statistical difference between the uptake of the KasB phosphomimetic strain compared to deletion strain (left panel). Complementation with a functional KasB restores the uptake of the phosphomimetic mutant (right panel). Blue bar, parental strain; green bar, KasB_T334A/T336A; black bar ΔkasB, black bar with white stripes, ΔkasB pMV261-kasB; red bar, KasB_T334D/T336D; and red bar with white stripes, KasB_T334D/T336D pMV261-kasB.