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. 2014 May 8;10(5):e1004125. doi: 10.1371/journal.ppat.1004125

Figure 1. EBV infection in vitro inhibits DOK1 gene expression.

Figure 1

Primary B-cells were isolated from healthy donor blood using negative selection, and then infected with GFP-EBV recombinant virus. (A) mRNA levels of EBNA1, LMP1, and DOK1 were measured using real time PCR at different time points 12, 16, 24, 36, and 48 hours post infection and normalized to GAPDH expression. The isolated primary B-cells were used as a control (time point 0). Data are average of three independent experiments. (B) DOK1, LMP1, EBNA1, and β-actin protein levels were determined by western blotting. (C) Total mRNA was extracted from RPMI, BJAB and Louckes cells two weeks after infection with GFP-EBV recombinant virus. The respective non-infected cells were used as control. Similarly, mRNA was extracted from two LCLs and their original primary B-cells. The expression levels of EBNA1, LMP1, and DOK1 were measured by real time PCR and normalized to GAPDH expression. (D) DOK1, LMP1 and β-actin protein levels were determined by western blotting. DOK1 protein levels were quantified from two independent immunoblots and normalized to the corresponding β-actin level (bottom of B and D).