Table 1. Primers and probes used for qPCR.
| Gene | Sequence | Accession number | Ref | |
| ELF α1 | Forward | GCCCCTCCAGGACGTTTA | AF321836 | [48] |
| Reverse | CGGCCCACAGGTACAGTT | |||
| Probe | CCAATACCACCGATTTTG | |||
| IFN type I | Forward | ACTGAAACGCTACTTCAAGAAGTTGA | AY216595, AY216594 | [48] |
| Reverse | GCAGATGACGTTTTGTCTCTTTCCT | |||
| Probe | CTGTGCACTGTAGTTCATTT | |||
| Mx1 and 2 | Forward | CCACCCTACAGGAGATGATGCT | U66475, U66476 | [20] |
| Reverse | ATCTGATCAGCCAAACGTTGACT | |||
| Probe | CTCAAGTCCTATTACAGGATATC | |||
| CD3γδ | Forward | TGATCACCGGCAAGAAAACATCT | EF421418, EF421419 | [70] |
| Reverse | TTTGCACTCGCCTCATTGC | |||
| Probe | ATGAGCCCCATTTTGCT | |||
| CD8α | Forward | AGAACGAAACGATGCCCACAT | AY693391, Y693393, AY701521 | [71] |
| Reverse | CTGTTGTTGGCTATAGGATGTTGTTG | |||
| Probe | TTCTCGGCTGTCTTTTG | |||
| MHC-I | Forward | GCGACAGGTTTCTACCCCAGT | AF504013-25 | [72] |
| Reverse | TGTCAGGTGGGAGCTTTTCTG | |||
| Probe | TGGTGTCCTGGCAGAAAGACGG | |||
| IL-1β | Forward | GCTCAACTTCTTGCTGGAGAGT | AY617117 | [48] |
| Reverse | GGGCGCCGACTCCAA | |||
| Probe | CTCCAACACTATATGTTCTTCC | |||
| IFNγ | Forward | GCTGTTCAACGGAAAACCTGTTT | AJ841811, AY795563 | [48] |
| Reverse | GTCCAGAACCACACTCATCCA | |||
| Probe | TCACTGTCCTCAAACGTG | |||
| IL-10 | Forward | CCGTTTGACATCAAC GAGTTCATCT | AB118099 | [73] |
| Reverse | CAGCTCTCCCATTGCCTTATACAG | |||
| Probe | CCTTATCCTGCATCTTCT | |||
| 18S | Forward | CCCCGTAATTGGAATGAGTACACTTT | AJ427629 | [39] |
| Reverse | ACGCTATTGGAGCTGGAATTACC | |||
| Probe | CTTTAACGAGGATCCATTGG | |||
| VP1 | Forward | CTGGTCCAGAAACCCTAAGAC | AY379740 | [43] |
| Reverse | GTGTGTATCTCTCCCCTTTTGG |
The table shows the primers and hydrolysis probes (FAM-MGB NFQ probes; Applied Biosystems/Life Technologies Ltd, Paisley, UK) used for qPCR analyses of mRNA expression. The primers used for SYBRGreen detection of the IPNV polymerase VP1 is shown in the last row.