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. 2014 May 8;9(5):e95738. doi: 10.1371/journal.pone.0095738

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HEK293T cells were transfected with NL4-3ΔVif plus A3G in the presence of WT or mutant Vif as indicated. After 48 h, HIV-1 viruses were harvested. (A) Virus infectivity was assessed using MAGI indicator cells, with the virus infectivity in the presence of WT Vif set to 100%. Error bars indicate the standard deviation from triplicate experiments. (B) A3G stability was assessed by immunoblotting against A3G-HA, Vif-HA and tubulin as a loading control. A3G packaging was evaluated by immunoblotting against A3G-HA and CAp24 after viruses were isolated by ultracentrifugation from the supernatants of cell cultures.