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. 2014 May 8;9(5):e96073. doi: 10.1371/journal.pone.0096073

Figure 1. Schematic representation of individual transgenes.

Figure 1

Nucleotide (nt) 3975 to 4928 region of the viral complementary (vc) strand of L RNA (yellow box) of Watermelon silver mottle virus (WSMoV) was amplified as the WLm fragment and constructed as translatable sense (pBGWLm), non-translatable sense (pBGWLmt and pBGWLmts), antisense (pBGWLmA) and inverted repeat (pBWLmhp) transgenes in the Ti binary vector pBI121. pBGWLmt and pBGWLmts contained identical sequence to pBGWLm, but two termination codons (italicized and asterisked) were added in-frame in pBGWLmt, and the same were added with a −1 frame shift in pBGWLmts. A linker sequence containing the tospoviral S RNA 3′-terminal consensus sequence (5′-ATTGCTCT-3′) and an NcoI site was added between antisense and sense WLm sequences for creating the inverted repeat in pBWLmhp. The restriction sites used for construction of transgenes are indicated. GUS leader sequence (GUS-L, green box), Cauliflower mosaic virus 35S promoter (35S-p, pink box) and nos terminator (NOS-t, purple box) are indicated.