Figure 7. Detection of transgene transcripts in R₀ lines of transgenic Nicotiana benthamiana and siRNA accumulation in R₂ progeny of N. bentamiana and tomato lines resistant to Watermelon silver mottle virus (WSMoV).

(A) All the analyzed resistant (R) lines showed lower levels of transgene transcripts than the susceptible (S) lines. The transgenic N. benthamiana lines completely resistant to 10 distinct tospovirus species are indicated by asterisks. Transgene transcripts were not detected in the NT plant of N. benthamiana. Ribosomal RNAs were used as loading controls. (B) siRNA accumulation of a susceptible (S) R₂ individual (WLmt 13-27-3) and a highly resistant (HR) R₂ individual (WLmt 13-27-12) of a tobacco line immune to WSMoV, in comparison to a non-transgenic (NT) plant, was analyzed by ³²P-labeled WLm-specific probe. (C) siRNA accumulation of R₂ progeny of a HR tomato line WLmhp 2. Accumulation of siRNA in R₂ HR individuals (WLmhp 2-2-3, 2-5-5 and 2-5-8), moderately resistant (MR) individuals (WLmhp 2-5-3, 2-2-22 and 2-5-23), an S individual (WLmhp 2-6-13), and a NT plant were analyzed by ³²P-labeled WLmhp-specific probe.