Table 3. Evaluation of the Watermelon silver mottle virus (WSMoV)-resistant transgenic Nicotiana benthamiana lines against other serologically unrelated tospovirus species, Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), Impatiens necrotic spot virus (INSV) and Groundnut chlorotic fan-spot virus (GCFSV) under greenhouse conditions.
| Transgene | Total assayed line No. | TSWV | GRSV | INSV | GCFSV | ||||||||
| HRa | MRb | Total R lines (%)c | HR | MR | Total R lines (%) | HR | MR | Total R lines (%) | HR | MR | Total R lines (%) | ||
| WLm | 14 | 0 | 6 | 6 (42.9) | 2 | 5 | 7 (50.0) | 2 | 3 | 5 (35.7) | 4 | 6 | 10 (71.4) |
| WLmt | 19 | 4 | 6 | 10 (52.6) | 4 | 6 | 10 (52.6) | 5 | 6 | 11 (57.9) | 7 | 7 | 14 (73.7) |
| WLmts | 19 | 7 | 4 | 11 (57.9) | 4 | 9 | 13 (68.4) | 7 | 6 | 13 (68.4) | 9 | 10 | 19 (100.0) |
| WLmA | 20 | 5 | 5 | 10 (50.0) | 6 | 5 | 11 (55.0) | 2 | 9 | 11 (55.0) | 5 | 10 | 15 (75.0) |
| WLmhp | 21 | 6 | 9 | 15 (71.4) | 2 | 13 | 15 (71.4) | 9 | 7 | 16 (76.2) | 6 | 10 | 16 (76.2) |
a
The lines that remained asymptomatic and ELISA negative for the N proteins beyond a month after inoculation were considered highly resistant (HR) lines.
b
The lines that showed more than 7 days delay in symptom development, in comparison to the non-transformed plants were classified as moderately resistant (MR) lines.
c
Both HR and MR lines were regarded as resistant (R) lines. The percentages of resistance were expressed as number of R lines/total number of assayed WSMoV-resistant lines.