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. 2014 May 8;9(5):e95400. doi: 10.1371/journal.pone.0095400

Figure 1. OxLDL and LPS synergize in the induction of the osteogenic responses in human AVICs.

Figure 1

Human AVICs were treated with LPS (0.10 µg/ml), oxLDL (20 µg/ml) or LPS plus oxLDL for 48 h. A. Representative immunoblots and densitometric data show that oxLDL up-regulates BMP-2 expression. Protein levels of BMP-2 are much higher in cells treated with LPS plus oxLDL than those in cells treated with LPS alone or oxLDL alone. B. OxLDL alone has a minimal effect on ALP levels, but cells treated with LPS plus oxLDL produce higher levels of ALP than cells treated with LPS alone. Similarly, ALP activity is higher in cells treated with LPS plus oxLDL. n = 4 separated cell isolates in each group, *P<0.05 vs. untreated control; †P<0.05 vs. cells treated with LPS alone or oxLDL alone.