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. 2014 May 8;9(5):e96298. doi: 10.1371/journal.pone.0096298

Figure 3. Confocal micrographs indicating bsaQ-dependent secretion of CHBP in U937 cells infected with B. pseudomallei.

Figure 3

PMA-activated U937 cells were separately infected with B. pseudomallei (K96243, bsaQ or chbP mutant strain). At different time points of infection (3, 6, 9 and 12 h), infected cells were stained using purified rabbit anti-CHBP antibody detected with anti-rabbit Ig-Alexa Fluor488 (Molecular Probes). The bottom panel shows the localization of CHBP and the top panel merges this signal with DIC images showing the position of infected cells. Scale bars, 10 µm.