Table 4. CCD with measured and predicted response of mRANKL production.

Run	A OD600	B Lactose (mM)	C Temperature (°C)	D Induction time (h)	Response mRANKL (mg/L)
					Predicted value	Actual value
1	−1	−1	−1	−1	16.7	15.9
2	1	−1	−1	−1	17.3	16.3
3	−1	1	−1	−1	16.8	17.5
4	1	1	−1	−1	26.8	27.9
5	−1	−1	1	−1	26.1	26.8
6	1	−1	1	−1	16.1	19.2
7	−1	1	1	−1	24.7	23.1
8	1	1	1	−1	23.9	23.9
9	−1	−1	−1	1	39.7	40.5
10	1	−1	−1	1	23.7	27.4
11	−1	1	−1	1	37.8	36.8
12	1	1	−1	1	31.1	31.3
13	−1	−1	1	1	41.8	42.8
14	1	−1	1	1	15.1	15.3
15	−1	1	1	1	38.3	40.2
16	1	1	1	1	20.9	23.8
17	−2	0	0	0	33.5	34.3
18	2	0	0	0	16.8	13.3
19	0	−2	0	0	39.1	36.9
20	0	2	0	0	45.1	44.6
21	0	0	−2	0	11.5	11.3
22	0	0	2	0	10.8	8.3
23	0	0	0	−2	15.7	16.2
24	0	0	0	2	35.8	32.6
25	0	0	0	0	49.5	49.7
26	0	0	0	0	49.5	50.6
27	0	0	0	0	49.5	48.7
28	0	0	0	0	49.5	47.8
29	0	0	0	0	49.5	48.9
30	0	0	0	0	49.5	51.6