Table 5. ANOVA for response surface quadratic model for mRANKL production.
| Source | df | Sum of Squares | Mean Square | F- Value | p-value Prob > F |
| Model | 14 | 5218.066 | 372.719 | 63.23285 | <0.0001 |
| A-OD600 | 1 | 420.8438 | 420.8438 | 71.39735 | <0.0001 |
| B-Lactose | 1 | 53.10375 | 53.10375 | 9.009204 | 0.0089 |
| C-Temperature | 1 | 0.84375 | 0.84375 | 0.143145 | 0.7105 |
| D-Induction time | 1 | 603.0038 | 603.0038 | 102.3013 | <0.0001 |
| AB | 1 | 86.02563 | 86.02563 | 14.59449 | 0.0017 |
| AC | 1 | 115.0256 | 115.0256 | 19.51443 | 0.0005 |
| AD | 1 | 276.3906 | 276.3906 | 46.89046 | <0.0001 |
| BC | 1 | 2.640625 | 2.640625 | 0.44799 | 0.5135 |
| BD | 1 | 4.100625 | 4.100625 | 0.695683 | 0.4173 |
| CD | 1 | 53.65562 | 53.65562 | 9.102831 | 0.0087 |
| A∧2 | 1 | 1016.091 | 1016.091 | 172.3827 | <0.0001 |
| B∧2 | 1 | 93.7686 | 93.7686 | 15.90811 | 0.0012 |
| C∧2 | 1 | 2520.691 | 2520.691 | 427.6424 | <0.0001 |
| D∧2 | 1 | 966.625 | 966.625 | 163.9907 | <0.0001 |
| Residual | 15 | 88.41583 | 5.894389 | ||
| Lack of Fit | 10 | 78.88083 | 7.888083 | 4.136383 | 0.0653 |
| Pure Error | 5 | 9.535 | 1.907 |
A = OD600; B = Lactose concentration; C = Temperature; D = Induction time; df = Degrees of freedom.