Figure 7. GA inhibits the growth of colorectal cancer and induces autophagy in vivo.

(A–E) C26 tumor-bearing mice were treated with hydroxypropyl-cyclodextrinvehicle (n  = 9) or GA 8 mg/kg (n = 9) twice daily by intraperitoneal injection for 12 days. (A) Excised xenografts showing tumor burden. (B) Mean tumor volume. (C) Tumor weight. (D) Immunohistochemistry analysis of LC3 and cleaved caspase 3 in tumor sections. (E) LC3 and cleaved caspase 3 expression from tumor lysates of three different mice were determined by immunoblot analysis. (F) Effect of 3-MA or NAC in combination with GA on growth of C26 tumor xenografts. Growth curve of tumor volumes started from 600 mm³.