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. 2014 May 8;9(5):e96593. doi: 10.1371/journal.pone.0096593

Figure 4. Expression of GLI1 target genes after GANT61 treatments in canine D17 cells.

Figure 4

Canine D17 OSA cells were treated with 12 µM of GANT61 for 96 hrs and relative expression was determined by Real Time RT-PCR. (A–C) GANT61 significantly decreased expression of GLI1, GLI2, and PTCH1 mRNA compared to untreated (UT) and vehicle DMSO treated cells. (D) Treatment of D17 cells with GANT61 significantly decreased expression of PAX6 mRNA. All mRNA expression studies were equilibrated with the housekeeping gene HPRT. Errors bars represent S.D., statistical analysis was performed using one-way ANOVA with post-hoc Tukey's test and significant values denoted as: * p<0.05, * * p<0.01, * * * p<0.001.