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. Author manuscript; available in PMC: 2015 Jun 27.
Published in final edited form as: J Biomech. 2013 Nov 8;47(9):2143–2148. doi: 10.1016/j.jbiomech.2013.10.051

Figure 3.

Figure 3

Viability of primary human chondrocytes in high concentration agarose gels after 24 and 72 hours. Primary human chondrocytes were harvested from joint replacement tissue (Bridger Orthopedic, Bozeman, MT), digested in Type IV collagenase (2 mg/mL for 12-14 hrs. at 37°C), cultured, encapsulated in physiologically-stiff agarose (3-5% w/v), and assessed for viability after 24 and 72 hours using confocal microscopy. Live cells were identified with Calcein AM fluorescence (ex. 497 em. 516) and dead cells with propidium iodide fluorescence (ex. 537 em. 617). All six gel locations were imaged showing minimal variability in cell viability throughout the gel. Average viability was >97% and >95% for 24 and 72 hour time periods, respectively.