Figure 2. Triplex formation of the modified TFOs in the presence of potassium. (A) Gel mobility shift analysis of triplex formation in standard triplex buffer (10mM MgCl2/ no K+). The target duplex was end-labeled and incubated with increasing concentrations of the indicated TFO followed by native PAGE. The lane marked 0 represents duplex DNA alone with no added TFO. (B) Gel mobility shift analysis of triplex formation in the presence of 140 mM KCl. (C) Gel mobility shift assay of AG30 in the presence of 140 mM KCl.