Figure 3. Potential effect of G-rich oligonucleotide self-aggregation on triplex formation. (A) CD spectra of AG30, A6G30, and A8G30 in standard triplex binding buffer (10 mM MgCl₂/no K⁺). All spectra were collected at room temperature in a species concentration of 2μM. (B) CD spectra collected in the presence of 140mM KCl. (C) Triplex formation using PPG-substituted TFOs at various time points. AG30, A6G30 and A8G30 (10^-8 M) were incubated with end-labeled target duplex (10^-10 M) for the indicated times in standard triplex binding buffer (10 mM MgCl₂/no K⁺).