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. 2014 May 8;9(5):e96832. doi: 10.1371/journal.pone.0096832

Figure 3. Transcriptional regulation patterns of genes determined by quantitative real-time PCR.

Figure 3

The RNA samples were purified from cultures of the wild-type (WT) and fur mutant and fur complemented strains under iron-replete (+Fe, grey bars) or iron-deplete (-Fe, white bars) conditions 1 h after addition of 100 µM iron or 150 µM desferal. The mRNA levels of fbpA and norB, genes that were repressed and activated by iron-bound Fur respectively, were used as controls for iron and Fur regulation in N. gonorrhoeae. The mRNA levels observed for the five conditions (WT strain under −Fe conditions, fur mutant strain under +Fe and −Fe conditions, and fur complemented strain under +Fe and −Fe conditions) were compared to the value of WT strain under +Fe conditions. The final results were represented as mean ± standard deviation. A * indicates significantly different compared to the mRNA level of WT+Fe. A ** indicates significantly different compared to the mRNA level of WT-Fe. The gene designations of N. gonorrhoeae F62 were assigned according to their homologues in N. gonorrhoeae FA1090.