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. 2014 May 8;9(5):e96917. doi: 10.1371/journal.pone.0096917

Figure 4. Generation of an rrp2 conditional lethal mutant in B. burgdorferi.

Figure 4

(A) Schematic representation of the bb0764–bb0761 genes in the B. burgdorferi chromosome and the insertion of PflgB-kan cassette into rrp2 by homologous recombination. Arrows indicate the approximate positions of the oligonucleotide primers used for subsequent analyses. (B) Analyses of the wild-type 297 and the rrp2 conditional lethal mutant OY179 by PCR. The specific primer pairs are indicated on the right. Lanes WT, 297; lanes M1, M2, and M3, three clones of OY179.