Table 2. MICs of Penicillin-G and identified mutations for transformants with increased resistance.
| Strain No. | Mutant PBPs | Mutagenesis strategy | 1MIC of Penicillin G (mg/L) | Relative MIC fold change | 2Identified mutations in PBPs |
| DA17021 | FtsI | Hydroxylamine | 12 | 2.4 | P311S |
| DA17023 | FtsI | Hydroxylamine | 13 | 2.6 | V545I |
| DA17025 | FtsI | Hydroxylamine | 8 | 1.6 | V377I |
| DA20073 | FtsI | ep-PCR | 22 | 4.4 | V545I, N579K, Syn N324 (AAC->AAT) |
| DA20075 | FtsI | ep-PCR | 24 | 4.8 | V545I, P50S |
| DA20078 | FtsI | ep-PCR | 14 | 2.8 | P311S, A49V, I159V |
| DA19904 | DacC | ep-PCR | 13 | 2.2 | M369L, Syn V374 (GTC->GTT), W384C |
| DA19906 | DacC | ep-PCR | 12 | 2 | S6Y, P199S, Syn A242 (GCT->GCA), Q365H, L368F |
| DA19908 | DacC | ep-PCR | 14 | 2.3 | F21L, S120T, Syn L330 (CTG->CTA) |
| DA19909 | DacC | ep-PCR | 16 | 2.7 | M45I, A104T, S147N, Syn S49 (AGC->AGT), Syn A141 (GCC->GCT) |
| DA19911 | DacC | ep-PCR | 13 | 2.2 | T92M, Syn D294 (GAC->GAT), G325W, |
| DA19912 | DacC | ep-PCR | 21 | 3.5 | Syn G116 (GGC->GGT), G146R, Syn L368 (TTA->TTG), M371I |
| DA20082 | DacB | ep-PCR | 10 | 1.7 | Syn A34 (GCC->GCT), M39V, V66E, P78L, Syn T85 (ACG->ACA), Syn T110 (ACG->ACA), Syn L123 (CTG->TTG) G127C, W151Stop, T157M, V192M, Syn S194 (TCT->TCG), T278M, Syn L298 (CTG->TTG), I318T, N350I, T351P, G356D, Syn R402 (CGC->CGG), A403T, Q455L, Y474Stop |
| DA20084 | DacB | ep-PCR | 8 | 1.3 | M1R, Q114L, K147Stop, R205Q, D312V, M371I |
1
MIC values were determined under 2 mM arabinose induction.
2
Syn = synonymous mutations.