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. 2014 Jun;85(6):849–857. doi: 10.1124/mol.113.090654

Fig. 7.

Fig. 7.

Effect of 17-DMAG and 19Ph-DMAG on Hsp90 client proteins and heat shock protein induction in human breast cancer cells is NQO1 dependent. Immunoblot analysis of Hsp90 client protein degradation (Raf-1 and Akt) and Hsp70 compensatory response in MDA468/NQ16 (A) and MDA468 cells (C) treated with 17-DMAG or 19Ph-DMAG at 5 µM for 24 hours. DMSO-treated cells served as control. Blots are representative of three independent experiments. (B and D) Fold change of the indicated proteins is normalized to DMSO control and estimated by densitometry. Data are expressed as mean ± S.E.M. (n = 3). *P < 0.05 versus DMSO; #P < 0.05 versus 17-DMAG (one-way analysis of variance with Tukey’s post-test).