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. 2014 Jun;85(6):849–857. doi: 10.1124/mol.113.090654

Fig. 8.

Fig. 8.

19Ph-DMAG inhibits growth in HER2-positive human breast cancer cells with molecular signatures of Hsp90 inhibition. (A) Growth inhibition following either 17-DMAG or 19Ph-DMAG treatment was measured by MTT analysis in BT474 cell lines. Data are expressed as mean ± S.E.M. (n = 3). (B) Immunoblot analysis of Hsp90 client proteins (HER2, Raf-1, and Akt) and Hsp70 compensatory response in BT474 cells treated with either 17-DMAG or 19Ph-DMAG at 5 µM for 24 hours. Blots are representative of three independent experiments. (C) Fold change of the indicated proteins is normalized to DMSO control and estimated by densitometry. Data are expressed as mean ± S.E.M. (n = 3) *P < 0.05 versus DMSO; #P < 0.05 versus 17-DMAG (one-way analysis of variance with Tukey’s post-test).