Fig. 1.
Discovery of VU0071063 in a Tl+ flux assay of Kir6.2/SUR1. (A) Plate map used in CRC analyses. DMSO (solvent) and broad-spectrum Kir channel inhibitor VU0573 (Raphemot et al., 2011) were used as controls. The 4-point test compound CRCs were distributed horizontally, whereas 11-point VU0573 CRCs were distributed vertically. (B) Fluorescence heat map recorded from the assay plate containing four doses of VU0071063 (red box). Fluorescence intensity is indicated by the pseudocolored scale (right), with cooler (blue) to hotter (red) colors corresponding to low high Tl+ flux, respectively. (C) Representative time versus normalized (F/F0) fluorescence intensity in wells containing the indicated concentrations of VU0071063.