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. 2014 Jun;85(6):858–865. doi: 10.1124/mol.114.091884

Fig. 2.

Fig. 2.

Characterization of VU0071063 activity against Kir6.2/SUR1 in Tl+ flux assays. (A) Chemical structures of VU0071063 (VU063), diazoxide (Diaz), and pinacidil (Pin). (B) Representative Tl+ flux experiment demonstrating activation of Kir6.2/SUR1 by 30 μM VU0071063 or 250 μM diazoxide, but not the vehicle control DMSO (0.3%). Fluorescence data have been normalized (F/F0) to baseline values recorded before Tl+ addition. (C) Dose-dependent activation of Kir6.2/SUR1 by VU0071063 and diazoxide, but not pinacidil (n = 4–6 independent experiments, each performed in triplicate). (D) Dose-dependent inhibition of VU0071063- and diazoxide-dependent Tl+ flux by glibenclamide (Glib) and tolbutamide (Tolb) (n = 3 independent experiments, each performed in triplicate).