Figure 1.

Expression of DAO mRNA in rat VTA and cerebellum. (A) Northern blot of rat cerebellar RNA showing that the DAO cRNA probe detects a single band of ~2 kb. (B) In situ hybridization, showing signal for DAO mRNA in the VTA, with clusters of grains over neurons (arrow). (C) VTA, sense strand hybridization control, showing low level of background signal. (D) Cerebellum, showing DAO mRNA concentrated in the Purkinje cell layer (P) with moderate signal in the granule cell layer (G) and low signal in the molecular layer (M). (E) Cerebellum, sense strand hybridization control. Sections are counterstained with cresyl violet.