Figure 2.

Monitoring mitochondrial localization of vMIA by confocal microscopy. Primary human foreskin fibroblasts (HFFs) lipofected with vectors expressing vMIA-EGFP and Mito-BFP were fixed with 4% paraformaldehyde (PFA) at 22 hours after transfection as described in the methods. (A) Images show a single optical plane for a cell expressing vMIA-EGFP (green) and Mito-BFP (pseudocolored red) imaged using confocal microscopy and (B) the same image plane following deconvolution of the entire Z-stack. (C,D) The boxed region of interest is enlarged on the right. (E) Intensity profile of vMIA-EGFP (green) and Mito‑BFP (red) emissions along the pixels marked by the dotted line on the deconvolved image are shown by the plot. For higher resolution images, see Supplemental Figure S1.