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. 2014 Apr 23;6(4):1861–1875. doi: 10.3390/v6041861

Figure 2.

Figure 2

EAex inhibited the influenza virus-induced pAKT pathway. Influenza A/WSN/33 (MOI: 0.1) was incubated with 0.025 mg/mL on ice for 1 h. The mixture was then used to infect A549 cells, which had been incubated overnight in serum-free medium. Cell lysates were harvested at 10, 20, 30, and 60 min after virus addition, and GAPDH, AKT, and pAKT (S473) were detected by immunoblotting. P: positive control; A549 cells were incubated in serum-free MEM for 24 h before treatment with 2% FBS in MEM for 1 h. These figures are representative of three independent, reproducible experiments. Relative pAKT expression was normalized with AKT, and then normalized to the mock infection at each time point.