Figure 1. The detection results based on different Mg²⁺ concentrations.

(A) LAMP products detected by 1,000× SYBR Green I. (B) Detection of LAMP products by agarose gel electrophoresis stained by EB (ethidium bromide). Tubes and lanes 1, 5, 9, 13, 17 and 21: ddH₂O. Tubes and lanes 2, 6, 10, 14, 18 and 22: FN95-1702 (negative control). Tubes and lanes 3, 4, 7, 8, 11, 12, 15, 16, 19, 20, 23 and 24: the plasmid 1Ac0229. Tubes and lanes 1–4, 5–8, 9–12, 13–16, 17–20 and 21–24: Concentration of Mg²⁺ is 4.50 mM, 4.75 mM, 5.00 mM, 5.25 mM, 5.50 mM and 5.75 mM, respectively. Lane M: DL 15,000+ 2,000 DNA marker.