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. 2014 May 9;4:4912. doi: 10.1038/srep04912

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(A) LAMP products detected by 1,000× SYBR Green I. (B) Detection of LAMP products by agarose gel electrophoresis stained by EB. Tubes and lanes 1, 5, 9 and 13: ddH₂O. Tubes and lanes 2, 6, 10 and 14: FN95-1702 (negative control). Tubes and lanes 3, 4, 7, 8, 11, 12, 15 and 16: the plasmid 1Ac0229. Tubes and lanes 1–4: ratio of inner and outer primers is 2: 1. Tubes and lanes 5–8: ratio of inner and outer primers is 4: 1. Tubes and lanes 9–12: ratio of inner and outer primers is 6: 1. Tubes and lanes 13–16: ratio of inner and outer primers is 8: 1. Lane M: DL 15,000+2,000 DNA marker.