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. 2014 May 9;4:4912. doi: 10.1038/srep04912

Figure 4. Sensitivity comparison of the LAMP assay and conventional PCR using the plasmid 1Ac0229 as template.

Figure 4

(A) LAMP products detected by 1,000× SYBR Green I. (B) Detection of LAMP products by agarose gel electrophoresis stained by EB. (C) PCR products detected by agarose gel electrophoresis stained by EB. Tube and lane 1: ddH2O. Tube and lane 2: FN95-1702 (negative control, CK). Tubes and lanes 3–15: plasmid 1Ac0229 copies of 4.31 × 109, 4.31 × 108, 4.31 × 107, 4.31 × 106, 4.31 × 105, 4.31 × 104, 4.31 × 103, 4.31 × 102, 4.31 × 101, 4.31 × 100, 4.31 × 10−1, 4.31 × 10−2 and 4.31 × 10−3, respectively. Lane M: DL 15,000+ 2,000 DNA marker.