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. 2014 May 9;4:4912. doi: 10.1038/srep04912

Figure 7. LAMP and conventional PCR detection results of seventeen putative cry1Ac transgenic sugarcane lines.

Figure 7

(A) LAMP products detected by 1,000× SYBR Green I. (B) Detection of LAMP products by agarose gel electrophoresis stained by EB. (C) PCR products detected by agarose gel electrophoresis stained by EB. Tube and lane 1: ddH2O. Tube and lane 2: FN95-1702 (negative control). Tube and lane 3: ROC22 (negative control). Tube and lane 4: the plasmid 1Ac0229 (positive control). Tubes and Lanes 5–21: the seventeen putative cry1Ac transgenic sugarcane in order of 16k-1, 16k-3, 16k-5, 16d-1, 16d-2, 16d-4, 16d-6, 19a-1, 19a-3, 19a-5, 19b-4, 20i-2, 20i-4, A-2, A-5, B-2 and B-5. Lane M: 100 bp DNA ladder.