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. 2014 May 9;4:4914. doi: 10.1038/srep04914

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(A) Comparison of mRNA levels of representative markers expressed in different tissues: Dj-ahnak, Dj-inx1, Dj-mhca; Dj-inx10; Dj-mmp1; Dj-mt-mmpA, Dj-ast4 and Dj-timp in berberine-treated planarians and in controls. H₂O: controls; B50: berberine 50 μM; B400: berberine 400 μM; B600: berberine 600 μM. In Real Time RT-PCR experiments, the expression levels are indicated in relative units, assuming as unitary the value of the controls. Each value is the mean ± s.d. of 3 independent experiments, carried out in 6 replicates. (B) Whole mount in situ hybridizations reveal the pattern of representative markers expressed in different tissues: Dj-ahnak, Dj-inx1, Dj-mhca, Dj-inx10; Dj-mmp1;Dj-mt-mmpA, Dj-ast4 and Dj-timp in 600 μM berberine-treated planarians (B600) and in controls (H₂O). Scale bar: 400 μm.