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Purity of AAV vector stocks. 1.6×1010 GC of AAV8 vector preparations (full: lot nos. V0083 and CS0010) and approximately 2.6×1010 empty particles (lot no. Z4033) were loaded onto an SDS–polyacrylamide gel; proteins were visualized by SYPRO ruby staining. The AAV capsid proteins (VP1, VP2, and VP3) are indicated. Lane 1, molecular weight marker; lane 2, lot no. V0083 (CsCl purified, full); lane 3, lot no. CS0010 (iodixanol purified, full); lane 4, lot no. Z4033 (CsCl, empty).
