FIG. 5.

Quantification of relative empty-to-full particle content in an iodixanol gradient-purified AAV8 vector preparation. (a) A full AAV8 vector preparation (5×10¹² GC) purified by iodixanol gradients (lot no. CS0010) was loaded onto a 0.34-ml CIM-QA disk and eluted with an 80–115 mM salt gradient. The y axis shows the absorbance (mAU) and the x axis the elution volume (ml). The detected conductivity and absorbance are represented by solid light and dark blue lines, respectively. Calculated baselines are represented as a red line. Peak retention volumes, peak areas, and GC content of fractions were determined as described in the legend to Fig. 4. (b) A mixture of 75 μl of empty AAV8 particles (lot no. Z4033) and 1×10¹² GC of lot no. CS0010 was run under the same conditions and the peaks were evaluated identically.