Table 1.

Viral Particle Titers and Percentage of Reference Values of the AAV2-RSS, Wild-Type AAVs, and In-House-Prepared Recombinant AAVs

AAV sample	Reference value	Mean (CBB/SDS–PAGE)	Stdv (CBB/SDS–PAGE)	Number of quantifications	Percent reference value	95% Confidence interval (percent reference value)
AAV2-RSS (at KC)	9.18×108a	1.08×109	3.99×108	5	118.1	80.0–156.1
AAV2-RSS (at MSSM)	9.18×108a	9.50×108	2.62×108	4	103.5	75.5–131.4
AAV2-RSS (KC and MSSM)	9.18×108a	1.03×109	3.30×108	9	111.6	87.9–135.2
AAV2 wild-type A	3.50×109b	2.72×109	4.47×108	4	77.6	65.1–90.1
AAV2 wild-type B	5.80×109b	5.34×109	1.46×109	4	92.0	67.3–116.8
AAV2 wild-type C	6.00×109b	5.29×109	1.17×109	4	88.2	69.1–107.2
AAV2 wild-type (A–C)	N/A	N/A	N/A	12	85.9	75.2–96.7
AAV9.GFP	3.28×109b	3.46×109	7.25×108	4	105.4	83.8–127.1
AAV1-SERCA	2.34×109b	2.48×109	1.89×108	3	106.1	97.0–115.3
AAV1-LacZ	3.91×109b	3.69×109	2.10×108	3	94.3	88.2–100.4
In house AAVs	N/A	N/A	N/A	10	102.3	93.2–111.4
All AAVs	N/A	N/A	N/A	31	98.7	89.6–107.7

AAV, adeno-associated virus; AAV2-RSS, AAV2 reference standard stock; CBB, Coomassie Brilliant Blue; GFP, green fluorescent protein; KC, virus analyzed at King's College London School of Medicine; LacZ, galactosidase; MSSM, virus analyzed at Mount Sinai School of Medicine; N/A, not applicable; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SERCA, sarco/endoplasmic reticulum calcium ATPase; stdv, standard deviation.

Note: Titers are expressed as viral particles per microliter. Rows in boldface represent the average of “Percent reference value” of all virus preparations of a particular type of virus, that is, AAV2-RSS, all wild-type AAVs, all recombinant AAVs prepared in-house, or all the virus preparations.

^aTiter determined by ELISA.

^bTiter determined by quantitative amino acid analysis.