FIG. 6.

HIV-1 challenge of CMAP1 HPC-derived macrophages. (A) HPC-derived macrophages, either nontransduced (NT) (♦), unpurified CMAP1-transduced (CMAP1-UP) (▪), or purified CMAP1 (CMAP1-P) (▲)-transduced, were challenged with an R5-tropic BaL-1 strain of HIV-1 at an MOI of 0.05. On various days postinfection, culture supernatants were analyzed for HIV-1 replication by p24 ELISA. (B) On day 28 post-infection, infected cultures were visualized by microscopy under 10× magnification. All experiments were performed in triplicate. Representative cell pictures are displayed.