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. 2012 Dec 17;24(1):1–10. doi: 10.1089/hgtb.2012.198

FIG. 3.

FIG. 3.

Restriction enzyme digestion of DNA isolated from HD Ad vectors. (a) Viral DNA was isolated and digested with BamHI. Constructs gAd.EF1αFABP5 and gAd.AlbLacZ showed the expected pattern of DNA fragments for vector and helper. However, gAd.DBD-shFABP5 (genome sizes 26.7 and 27.2 kb), gAd.DBD-shSCR, gAd.DBD-CPT1AWT, gAd.DBD-CPT1E3A, and gAd.EF1αLacZ showed unexpected DNA fragments (arrows). (b) PCR fragments obtained using primers described in Figure 4, to amplify specifically from: (i) packaging signal of the HD Ad and the E1 sequence, and (ii) H14 E4 sequence and packaging signal of the HD Ad.