FIG. 2.
Neutral agarose gel electrophoresis and Southern blot examination of the isolated vector genomes from WT and serine-mutant AAV3 vectors. Equivalent amounts of DNA samples from the WT and serine-mutant AAV3 vectors (based on DNA slot-blots in Fig. 1) were isolated and analyzed on Southern blots. S663V vector genomes were loaded in twofold dilutions (left two lanes). The membranes were analyzed using 32P-labeled DNA probes specific to (A) CBAp or (B) SV40 Poly (A) sequences. The numbers in parentheses represent vector titers determined by Southern blot assays.