FIG. 3.
Native agarose gel electrophoresis and Southern blot analyses of the isolated genomes from WT and serine-mutant scAAV3 vectors. (A) Schematic structure of the scAAV-AFPp-EGFP vector genome. The size of the entire viral genome is 1.8 kb. (B) A representative native agarose electrophoresis and Southern blot examination of the isolated AAV genome. The hybridizations were analyzed with 32P-labeled DNA probes specific to (B) the AFPp or (C) the SV40 Poly (A) sequences. Color images available online at www.liebertpub.com/hgtb