FIG. 5.
Characterization of oversized scAAV-CBAp-Luc and scAAV-HS2-βp-globin genomes by DNA slot-blot assays. Schematic structures of (A) the scAAV-CBAp-Luc, and (D) the scAAV-HS2-βp-globin vector genomes. The sizes of the entire viral genome are 3.0 kb and 3.4 kb, respectively. Representative DNA slot-blot analysis of the isolated AAV genomes from scAAV2, scAAV3, and scAAV8 serotype vectors. The membranes were hybridized with the indicated 32P-labeled DNA probes specific to (B, F) the sequences located in the middle of the vector genomes or to (C, E) the sequences located at the ends of the vector genomes. The indicated amounts of the recombinant plasmids for each vector genome were used as appropriate controls. Results in each panel are representative of two independent experiments. The vector titers determined by Southern blots are indicated in parentheses. Color images available online at www.liebertpub.com/hgtb