. 2012 Jun 26;23(3):213–224. doi: 10.1089/hgtb.2012.074

Table 2.

Results of Magnetic-Activated Cell Sorting of T Cells with Peptide–MHC Multimers or CD34 Monoclonal Antibody: In Vitro Evaluation^a

	TCR T cells			TCR-tCD34 T cells
	T	P	S	S	CD34
T cell output numbers (directly after MACS)^b	±	–	+	+	++
T cell yield (1 week after MACS)^c	±	–	+	±	++
T cell expansion (1 week after MACS)^d	±	±	++	–	++
T cell expansion (3–6 weeks after MACS)^d	±	+	+	+	++
pMHC binding (1 week after MACS)^e	+	+	++	+	–
pMHC binding (3–6 weeks after MACS)^e	+	–	++	±	–
tCD34 expression (1 week after MACS)^f	NA	NA	NA	++	++
tCD34 expression (3–6 weeks after MACS)^f	NA	NA	NA	++	++
Antigen-specific CD107a mobilization^g	ND	ND	±	±	++
Antigen-specific IFN-γ secretion^h	ND	ND	±	±	++

^{^a}

Primary human T cell populations were transduced with TCR or TCR-tCD34 genes, MACSorted with gp100/A2 tetramers (T), pentamers (P), or Streptamers (S), or with CD34 mAb (CD34), and evaluated for various in vitro parameters. See Materials and Methods for details.

^{^b}

T cell numbers directly after MACS (counted microscopically): –, 0–0.10×10⁶:±, 0.10–0.25×10⁶;+, 0.26–0.75×10⁶; ++, >0.75×10⁶.

^{^c}

T cell numbers 1 week after MACS (counted microscopically): –, 0–1×10⁶;±, 2–5×10⁶; +, 6–15×10⁶; ++, >15×10⁶.

^{^d}

T cell expansion 1 or 3–6 week(s) after MACS (fold increase in T cell numbers at 1 week vs. directly after MACS and 3–6 weeks vs. 1 week after MACS, respectively): –, 0–10;±, 11–15;+, 16–20; ++, >20.

^{^e}

Peptide–MHC binding 1 and 3–6 week(s) after MACS (measured by flow cytometry with gp100/A2 pentamer): –, ≤20% (value before MACS);±, 21–30; +, 31–50; ++, >50%.

^{^f}

tCD34 expression 1 and 3–6 week(s) after MACS (measured by flow cytometry with CD34 mAb):–, ≤70% (value before MACS);±, 71–80; +, 81–90; ++, >90%.

^{^g}

T cell CD107a mobilization 3–6 weeks after MACS (measured by flow cytometry with CD107a mAb on peptide-specific stimulation): –, 0–20;±, 21–40; +, 41–60; ++, >60%.

^{^h}

T cell IFN-γ production 3–6 weeks after MACS (measured by ELISA on peptide-specific stimulation): –, 0–5;±, 6–10; +, 11–20; ++, >20 ng/ml.

MACS, magnetic-activated cell sorting; NA, not applicable; ND, not determined; pMHC, peptide–MHC; tCD34, truncated CD34.