Table 3.
Chemokine expression in various organs from control and APP-infected pigs.
|
Bone marrow |
Lungs |
Tracheobronchial LN |
||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Control |
APP-infected |
Control |
APP-infected |
Control |
APP-infected |
|||||||||||||||||||
| Unaffected area | Demarcation zone | Necrotic area | ||||||||||||||||||||||
| CCL1 |
0.028 |
± |
0.018 |
0.050 |
± |
0.037 |
0.025 |
± |
0.006 |
0.013 |
± |
0.002 |
0.008 |
± |
0.002* |
0.152 |
± |
0.055* |
0.009 |
± |
0.001 |
0.007 |
± |
0.001 |
| CCL2 |
0.195 |
± |
0.043 |
0.486 |
± |
0.183 |
1.799 |
± |
0.291 |
2.027 |
± |
0.394 |
2.785 |
± |
0.831 |
101.809 |
± |
25.084** |
3.831 |
± |
0.228 |
5.296 |
± |
1.162 |
| CCL3 |
0.117 |
± |
0.015 |
0.174 |
± |
0.026* |
0.623 |
± |
0.121 |
2.149 |
± |
0.497** |
1.521 |
± |
0.313* |
148.299 |
± |
48.339** |
0.240 |
± |
0.057 |
1.169 |
± |
0.307** |
| CCL4 |
0.103 |
± |
0.017 |
0.148 |
± |
0.024 |
0.805 |
± |
0.108 |
2.006 |
± |
0.451* |
1.506 |
± |
0.458 |
57.529 |
± |
15.897** |
0.291 |
± |
0.057 |
0.922 |
± |
0.158** |
| CCL5 |
0.015 |
± |
0.014 |
0.054 |
± |
0.000 |
2.610 |
± |
0.455 |
2.015 |
± |
0.322 |
1.259 |
± |
0.210 |
11.351 |
± |
1.899** |
2.293 |
± |
0.481 |
1.540 |
± |
0.179 |
| CCL8 |
0.099 |
± |
0.013 |
0.158 |
± |
0.024* |
0.121 |
± |
0.023 |
0.110 |
± |
0.016 |
0.125 |
± |
0.012 |
0.428 |
± |
0.153 |
0.336 |
± |
0.080 |
0.380 |
± |
0.038 |
| CCL11 |
0.003 |
± |
0.001 |
0.001 |
± |
0.000 |
0.002 |
± |
0.001 |
0.004 |
± |
0.002 |
0.007 |
± |
0.003 |
0.132 |
± |
0.032** |
0.002 |
± |
0.001 |
0.006 |
± |
0.004 |
| CCL17 |
0.000 |
± |
0.000 |
0.001 |
± |
0.000 |
0.033 |
± |
0.012 |
0.025 |
± |
0.006 |
0.021 |
± |
0.005 |
0.120 |
± |
0.031 |
0.715 |
± |
0.085 |
0.430 |
± |
0.080* |
| CCL19 |
0.183 |
± |
0.039 |
1.478 |
± |
0.690** |
1.526 |
± |
0.097 |
3.689 |
± |
0.540** |
3.813 |
± |
0.728* |
39.163 |
± |
9.875** |
45.957 |
± |
4.607 |
77.840 |
± |
7.874* |
| CCL20 |
0.000 |
± |
0.000 |
0.001 |
± |
0.000 |
0.009 |
± |
0.002 |
0.010 |
± |
0.002 |
0.019 |
± |
0.003 |
6.833 |
± |
1.846** |
0.288 |
± |
0.046 |
0.736 |
± |
0.217 |
| CCL21 |
0.016 |
± |
0.008 |
0.006 |
± |
0.001 |
0.976 |
± |
0.107 |
1.053 |
± |
0.166 |
1.147 |
± |
0.189 |
0.533 |
± |
0.138* |
17.522 |
± |
2.483 |
6.464 |
± |
2.130** |
| CCL22 |
0.003 |
± |
0.001 |
0.002 |
± |
0.001 |
0.038 |
± |
0.013 |
0.024 |
± |
0.006 |
0.024 |
± |
0.004 |
0.153 |
± |
0.040* |
0.673 |
± |
0.124 |
0.306 |
± |
0.049* |
| CCL25 |
0.007 |
± |
0.001 |
0.006 |
± |
0.001 |
0.013 |
± |
0.001 |
0.011 |
± |
0.001 |
0.013 |
± |
0.001 |
0.011 |
± |
0.001 |
0.010 |
± |
0.001 |
0.006 |
± |
0.001** |
| CCL27 |
0.000 |
± |
0.000 |
0.001 |
± |
0.000 |
0.002 |
± |
0.000 |
0.003 |
± |
0.000 |
0.002 |
± |
0.000 |
0.005 |
± |
0.002 |
0.001 |
± |
0.000 |
0.001 |
± |
0.000 |
| CCL28 |
n.d. |
n.d. |
0.036 |
± |
0.003 |
0.038 |
± |
0.013 |
0.045 |
± |
0.032 |
0.011 |
± |
0.003** |
n.d. |
n.d. |
||||||||
| CXCL9 |
0.180 |
± |
0.026 |
0.141 |
± |
0.018 |
1.704 |
± |
0.180 |
1.186 |
± |
0.288 |
0.820 |
± |
0.197* |
1.644 |
± |
0.740 |
4.188 |
± |
0.591 |
1.790 |
± |
0.225** |
| CXCL10 |
0.983 |
± |
0.222 |
0.996 |
± |
0.197 |
5.272 |
± |
0.425 |
3.685 |
± |
0.742 |
2.788 |
± |
0.545* |
8.892 |
± |
4.605 |
6.644 |
± |
0.826 |
3.097 |
± |
0.531** |
| CXCL11 |
0.353 |
± |
0.209 |
0.036 |
± |
0.013 |
0.124 |
± |
0.037 |
0.072 |
± |
0.023 |
0.059 |
± |
0.017 |
0.457 |
± |
0.201 |
0.146 |
± |
0.038 |
0.030 |
± |
0.007** |
| CXCL12 |
1.061 |
± |
0.123 |
1.022 |
± |
0.224 |
0.378 |
± |
0.047 |
0.241 |
± |
0.034* |
0.246 |
± |
0.040 |
0.453 |
± |
0.081 |
6.123 |
± |
0.842 |
2.024 |
± |
0.580** |
| CXCL14 |
0.794 |
± |
0.075 |
1.376 |
± |
0.322 |
1.935 |
± |
0.241 |
2.553 |
± |
0.381 |
4.312 |
± |
0.547* |
35.412 |
± |
4.900** |
2.961 |
± |
0.400 |
5.579 |
± |
0.586** |
| CXCL16 |
0.605 |
± |
0.058 |
0.372 |
± |
0.036* |
3.371 |
± |
0.211 |
2.340 |
± |
0.106** |
2.639 |
± |
0.343* |
9.151 |
± |
0.881** |
1.427 |
± |
0.092 |
2.510 |
± |
0.486** |
| CX3CL1 | 0.010 | ± | 0.004 | 0.025 | ± | 0.006 | 0.078 | ± | 0.017 | 0.044 | ± | 0.011 | 0.050 | ± | 0.010 | 0.639 | ± | 0.225* | 0.106 | ± | 0.008 | 0.048 | ± | 0.016* |
The data are shown as a fold change of chemokine mRNA expression in relation to the reference gene HPRT as measured by the RT-qPCR. Data are expressed as mean ± S.E.M. (Control: n = 6, APP-infected: n = 9). The significant differences (Mann–Whitney U test) between control and APP-infected pigs are marked with *P < 0.05, **P < 0.01. The values which were elevated between control and APP-infected pigs are highlighted in bold. LN, lymph node, n.d, non-detected.