Fig. 5.
Down-regulation of SOX4 mRNA expression by miR-203 (A), miR-335 (B) and miR-618 (C). Cancer cells (Ishikawa) underwent transient transfection with miR-203, miR-335, or miR-618 at the indicated concentrations for 24 h. Gene expression was determined by RT-qPCR analysis and compared to untreated controls. GAPDH was used as an internal control gene. Error bar, SD; and *, P<0.05 compared with untreated cells. (D) A proposed model of miR-203 and SOX4 interactions in normal and cancer cells.