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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Mar 10;38(5):1275–1283. doi: 10.1111/acer.12379

Figure 3. Relative Luciferase activity associated with Crhr2 promoter polymorphism.

Figure 3

(A) Three pGL-3 promoter plasmids (pGL3-P, pGL3-NP and pGL3 basic) were transfected into SK-N-SH cells to determine whether the Crhr2 promoter polymorphism affects luciferase gene expression. The relative expression of the luciferase gene from each construct is depicted as the mean ± SEM. ANOVA was used to detect significant differences in mean values within and between multiple constructs (p<0.05). (B) Using Genomatix software, the predicted transcription factor binding sites included CREB, C2H2, and Pax3.