Fig. 9.

Expression of 24-hydroxylase in the cortex. Cryosections were prepared from female wild-type (WT) and 24-hydroxylase knockout (KO) mouse brains and stained with the monoclonal antibody 1A7, and immunocomplexes were visualized by DAB-HRP staining. 24-Hydroxylase was detected in the somata of cortical neurons within layers II/III, V, and VI but not in nonpyramidal neurons of layer IV or those of layer I (A). A diffuse staining of the neuropil was present in all layers of the cortex. No staining was visible in cortical sections from knockout brains (B). Scale bar = 100 μm.