Table 3.
Summary of mRNA expression in TXhfq (in comparison with that in TX01) as determined by qRT-PCR
| Spo no. | Description | Protein level (by 2-DE) | mRNA level (by qRT-PCR) |
|---|---|---|---|
| 1 |
rpsA gene product [Edwardsiella tarda EIB202]: 30S ribosomal protein S1 |
Down (0.42 ± 0.05) |
Unchanged |
| 2 |
hypothetical protein1 [Edwardsiella tarda EIB202]: |
Down (-∞) |
Down (0.38 ± 0.06) |
| 3 |
tuf2 gene product [Edwardsiella tarda EIB202]: elongation factor Tu |
Down (0.35 ± 0.09) |
Unchanged |
| 4 |
Putative carbamoyltransferase YgeW [Edwardsiella tarda ATCC 23685] |
Down (0.21 ± 0.12) |
Down (0.42 ± 0.09) |
| 5 |
Mrp(Multiple Resistance and pH adaptation) protein [Edwardsiella tarda FL6-60] |
Up (2.81 ± 1.11) |
Up (4.11 ± 0.85) |
| 6 |
tsf gene product [Edwardsiella tarda EIB202]: translation elongation factor Ts |
Down (0.45 ± 0.07) |
Unchanged |
| 7 |
Dyp-type peroxidase family protein [Edwardsiella tarda EIB202] |
Up (3.62 ± 0.78) |
Up (7.72 ± 1.08) |
| 8 |
ybaS gene product [Edwardsiella tarda EIB202]: glutaminase |
Down (-∞) |
Down (0.20 ± 0.07) |
| 9 |
Hypothetical protein [Edwardsiella tarda EIB202]: lysophospholipase |
Up (3.21 ± 1.28) |
Up (5.10 ± 0.84) |
| 10 |
fkpA gene product [Edwardsiella tarda EIB202]: FKBP-type peptidyl-prolyl cis-trans isomerase |
Up (4.24 ± 1.35) |
Up (7.54 ± 1.21) |
| 11 |
ompR gene product [Edwardsiella tarda EIB202]: osmolarity response regulator; cytoplasmic |
Down (-∞) |
Down (0.18 ± 0.04) |
| 12 |
iscU gene product [Edwardsiella tarda EIB202]: FeS cluster assembly scaffold |
Up (2.23 ± 0.62) |
Unchanged |
| 13 |
Uncharacterized membrane protein YqjD [Edwardsiella tarda FL6-60] |
Down (0.25 ± 0.04) |
Down (0.54 ± 0.05) |
| 14 |
Putative iron-containing alcohol dehydrogenase [Edwardsiella tarda FL6-60] |
Down (0.27 ± 0.06) |
Unchanged |
| 15 | Cysteine synthase [Edwardsiella tarda C07-087] | Down (0.46 ± 0.05) | Down (0.33 ± 0.08) |