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Antibody responses to recombinant NY-ESO-1 protein as determined by ELISA. Sera from 13 patients in Cohort 1 (100-μg dose) and 12 patients in Cohort 2 (200-μg dose) were collected at each vaccination, and serially diluted by 400, 1,600, 6,400, 25,600 and 102,400. Reciprocal titers were determined based on the maximally diluted sera, which showed a higher OD (optical density of 450–500) value than the cut-off level for NY-ESO-1 antibody positivity.
