Table 7.
Secreted/soluble angiotensin-converting enzyme 2 (ace2) levels in culture media of human cardiomyocytes under normoxia and hypoxia with or without insulin treatment
|
Group |
Normoxia |
Hypoxia |
||
|---|---|---|---|---|
| +Insulin | -Insulin | +Insulin | -Insulin | |
| VC |
4.2±1.4 |
0.8±0.4 |
19.2±5.3* |
1.0±0.5 |
| WT-IRS-1 |
4.5±1.2 |
0.9±0.6 |
20.7±5.5* |
1.3±0.7 |
| Arg972-IRS-1 |
3.8±1.4 |
0.6±0.4 |
9.3±3.2*,a,b |
0.8±0.3 |
| VC+LY |
0.5±0.4 |
0.5±0.3 |
0.5±0.3a,b,c |
0.5±0.4 |
| SC |
4.0±1.1 |
0.8±0.3 |
20.3±5.6* |
1.1±0.6 |
| IRS-1-shRNA | 0.6±0.3 | 0.5±0.4 | 0.7±0.5a,b,c | 0.6±0.4 |
Note: Human cardiomyocytes were stably transfected with wild type (WT) IRS-1 or Arg972 IRS-1, or stably transduced with IRS-1-shRNA. Then the cells were cultured under normoxia or hypoxia for 24 hours in the presence or absence of LY294002 (LY, 50 μM) or insulin (1 mg/L). Human cardiomyocytes stably transfected with empty pcDNA3.1 plasmids was used as a control (VC). Secreted/soluble ACE2 levels (ng/mL) in the cell culture media were determined by ELISA. *p<0.05 compared with any group under normoxia; ap<0.05 compared with VC or SC under hypoxia+insulin (1 mg/L); bp<0.05 compared with WT-IRS-1 under hypoxia+insulin (1 mg/L); cp<0.05 compared with Arg972-IRS-1 under hypoxia+insulin (1 mg/L).