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. 2014 Apr;20(2):171–184. doi: 10.5056/jnm.2014.20.2.171

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© 2014 The Korean Society of Neurogastroenterology and Motility

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Transcriptional changes in muscarinic (M₂ and M₃) and neurokinin (NK₁ and NK₂) receptor expression in the W/W^V gastric fundus. (A) Representative gels displaying amplification products from gastric fundus-derived RNA using gene specific primers for Kit, Chrm2, Chrm3 (M₂ and M₃ receptors), Tacr1 and Tacr2 (NK₁ and NK₂ receptors) in wild-type and W/W^V animals. Note that the polymerase chain reaction (PCR) gel is not quantitative. (B) Quantitative PCR (q-PCR) revealed decreased levels of Kit transcripts in W/W^V fundus compared to wild-type (^**P < 0.001). (C) q-PCR showed that Tacr1 (NK₁ receptor) expression was not significantly different between wild-type and W/W^V mice, whereas Tacr2 (NK₂) was significantly reduced in W/W^V fundus (^**P < 0.001). (D) Chrm2 (M₂ receptors) showed significant reduction in their expression in W/W^V muscles (^*P < 0.05). (E) Chrm3 (M₃) showed a significant increase W/W^V fundus compared to wild-type muscles (^**P < 0.001). Hypoxanthine guanine phosphoribosyl transferase (HPRT) was used as a house-keeping gene. NS, not significant.